Protection of avian myeloblastosis virus (AMV) DNA polymerase by substrates against heat inactivation
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چکیده
منابع مشابه
Protection of avian myeloblastosis virus (AMV) DNA polymerase by substrates against heat inactivation.
Purified DNA polymerase from AMV was exposed to thermal inactivation in the presence and absence of various compounds required for maximal activity. A synergistic protective effect was noted when all the components were present. The enzyme showed a distinct preference for primed templates, especially for its native 70S RNA. Heat inactivation studies also suggested that the active sites for RNas...
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PYRAN COPOLYMER, A KNOWN IMMUNOSTIMULATOR, WAS FOUND TO BE A POTENT INHIBITOR OF PURIFIED DNA POLYMERASE (DEOXYNUCLEOSIDETRIPHOSPHATE: DNA deoxynucleotidyltransferase; EC 2.7.7.7) isolated from avian myeloblastosis virus. Unlike other inhibitors, pyran showed unique features of inhibition. It interacts with the polymerase at a region other than the template site. The inhibitory effect was overc...
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A recent report (1) presented evidence for allosterism in reverse transcription by Mason-Pfizer monkey virus reverse transcriptase and by E. coli DNA polymerase I. Our experiments also demonstrate these apparent cooperative effects when synthesis is catalyzed by either avian myeloblastosis virus DNA polymerase, feline sarcoma virus DNA polymerase, or E. coli DNA polymerase I (large fragment). W...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1974
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/1.11.1399